Experimental study of T lymphocyte reactivity inhibited by allogeneic bone marrow mesenchymal stem cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 740-742, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-244006
ABSTRACT
<p><b>OBJECTIVE</b>To address the question whether bone marrow mesenchymal stem cells (MSCs) could lower responsiveness of allogeneic T lymphocytes against alloantigens, and explore a feasible strategy for prevention of graft versus host disease (GVHD) occurred in allogeneic bone marrow transplantation.</p><p><b>METHODS</b>T cells were co-cultured with (60)Co-irradiated bone marrow MSCs from different individuals. The proliferative activity of T cells and their reactivity to allogeneic cells and ConA were evaluated with (3)H-TdR incorporation assay.</p><p><b>RESULTS</b>T cells could not be activated upon primary or even secondary exposure to allogeneic MSCs (compared the CPM value of 27,529 +/- 969 of T cell alone with that of primary and secondary exposures to allogeneic MSCs were 9,126 +/- 654 and 13,260 +/- 874, respectively). When MSCs were induced to express HLA-DR, they still could not elicit T cell activation. The proliferation rate of allogenous T cells exposed to MSCs was dramatically declined when T cells from the same donor's MSCs were used as stimulator (CPM value decreased from 45,876 +/- 5285 before coculture to 9850 +/- 1618 after coculture). Furthermore, the results remained unchanged even ConA was added into the culture system.</p><p><b>CONCLUSIONS</b>Heterogenetic MSCs could suppress T cell activation. MSCs pretreatment might be useful in the prevention of GVHD in HLA-mismatched bone marrow transplantation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow Cells
/
Lymphocyte Activation
/
T-Lymphocytes
/
Cell Communication
/
Cells, Cultured
/
Coculture Techniques
/
Cell Proliferation
/
Allergy and Immunology
/
Mesenchymal Stem Cells
/
Graft vs Host Disease
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS