miRNA changes in the reverse remodeling heart of rats / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 745-750, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-244174
ABSTRACT
<p><b>OBJECTIVE</b>To establish a reverse remodeling heart model in rats and observe collagen and TGF-β expression and relevant microRNAs changes during reverse remodeling.</p><p><b>METHODS</b>Lewis rats were divided into four groups including sham (NL, n = 10), abdominal aortic constriction (AAC, n = 10), heterotopic transplantation of abdominal aortic constriction (AAC-HT, n = 9) and heterotopic transplantation of normal heart (HT, n = 8). Left ventricular wall thickness and LV cavity were measured by echocardiography. The cardiomyocyte cross-sectional area (CSA) was determined on HE stained sections. Immunohistochemical and qRT-PCR were used to detect collagen and TGF-β expressions. miRNAs were detected by MicroRNA microarray.</p><p><b>RESULTS</b>Heart weight, left ventricular wall thickness and CSA were significantly increased in AAC hearts compared to those in the NL and AAC-HT hearts. The collagen and TGF-β were increased in AAC hearts and further increased in AAC-HT hearts. miRNA microarray evidenced more than two folds changes on 82 miRNAs compared to NL (10 in AAC, 32 in AAC-HT and 40 in HT).</p><p><b>CONCLUSION</b>Rat abdominal aortic constriction and heterotopic transplantation could be used as a reverse remodeling heart model and significant collagen and TGF-β as well microRNA expression changes were evidenced in this model.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rats, Inbred Lew
/
Transforming Growth Factor beta
/
Collagen
/
Ventricular Remodeling
/
Myocytes, Cardiac
/
MicroRNAs
/
Heart Ventricles
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2010
Type:
Article
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