Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2741-2745, 2012.
Article
in English
| WPRIM
| ID: wpr-244363
ABSTRACT
<p><b>BACKGROUND</b>Over-expression of P-glycoprotein (P-gp), encoded by the MDR1 gene, confers multidrug resistance (MDR) in renal cell carcinoma (RCC) and is a major reason for unsuccessful chemotherapy. This study aimed to determine the effct of RNA interference (RNAi) on the reversal of MDR in human RCC.</p><p><b>METHODS</b>We designed and selected one short hairpin RNA (shRNA) targeting MDR1 gene, which is stably expressed from integrated plasmid and transfected by lentivirus fluid in human RCC A498 cell.</p><p><b>RESULTS</b>The MDR1-targeted RNAi resulted in decreased MDR1 gene mRNA level (P < 0.001), almost abolished P-gp expression and reversed MDR to different chemotherapy drugs in the RCC A498 cell line.</p><p><b>CONCLUSION</b>MDR could be reversed by RNAi in human RCC A498 cell line, which may be used for clinical application in future.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Carcinoma, Renal Cell
/
Lentivirus
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Inhibitory Concentration 50
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Cell Proliferation
/
Genetics
/
Metabolism
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2012
Type:
Article
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