Study on morphology of surface atoms conformation of nanogold-based genechip / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1214-1217, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-244659
ABSTRACT
Conformations of surface atoms in various stages of nanogold-based genechip testing were scanned by the atomic force microscope based on the scanning tunneling microscope. The findings were First, the surface atoms of genechip slide (formylphenyl glass) were in a regular porous-arrangement; Second, after combination with probe, the regular porous arrangement changed to be irregular; Third, after hybridization with the target nucleic acid, the surface atoms were once again in a cable-like arrangement which was relatively structured and intensively cross-parallel. However, after the silver staining, the surface atoms showed a larger block structure with serious unevenness. From these results we can intuitively know the process and differences in probe combination, nucleic acid hybridization, and silver staining. Moreover, the relevant experiment was verified at the micro-level.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Particle Size
/
Surface Properties
/
Chemistry
/
Microscopy, Scanning Tunneling
/
Microscopy, Atomic Force
/
Oligonucleotide Array Sequence Analysis
/
Nanotechnology
/
Metal Nanoparticles
/
Gold
/
Methods
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Type:
Article
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