Expression of mRNA of GATA-3 and T-bet in the peripheral blood mononuclear cells of atopic asthmatics / 대한내과학회지
Korean Journal of Medicine
;
: 67-74, 2004.
Article
in Korean
| WPRIM
| ID: wpr-24474
ABSTRACT
BACKGROUND:
It is well known that the expression of Th2 cytokines are up-regulated in the atopic asthma. The study was to investigate the expression of transcription factors, such as GATA-3, c-maf, T-bet which are known to be involved in the T cell differentiation in the peripheral blood mononuclear cells (PBMCs) of atopic asthmatics.METHODS:
PBMCs were obtained from non-atopic controls and atopic asthmatics and cultured for 48 hours, and then 12-o-tetracanoylphorbol-13-acetate (PMA) and calcium ionophore (ionomycin) were added. mRNA of GATA-3, c-maf, T-bet, IL-4, IL-5, IL-13 and IFN-gamma were measured by RT-PCRRESULTS:
mRNAs of Th2 cytokines, such as IL-4, IL-5 and IL-13 were expressed higher in the atopic asthmatics, and that of Th1 cytokine, IFN-gamma was expressed higher in the non-atopic controls. GATA-3 and c-maf were expressed higher and T-bet was expressed lower in the atopic asthmatics. Expressions of GATA-3, c-maf and T-bet were not changed with the stimulation of PMA/Ionomycin.CONCLUSION:
This study suggest that GATA-3, c-maf and T-bet participate in the Th2 type inflammation in atopic asthmatics, like GATA-3 and c-maf as stimulatory factors, and T-bet as inhibitory factor.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Transcription Factors
/
RNA, Messenger
/
Cell Differentiation
/
Calcium
/
Cytokines
/
Interleukin-4
/
Interleukin-5
/
Interleukin-13
/
Inflammation
Language:
Korean
Journal:
Korean Journal of Medicine
Year:
2004
Type:
Article
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