Production of a fragment of glycoprotein G of herpes simplex virus type 2 and evaluation of its diagnostic potential
Singapore medical journal
;
: 346-352, 2015.
Article
in English
| WPRIM
| ID: wpr-244781
ABSTRACT
<p><b>INTRODUCTION</b>Herpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit.</p><p><b>METHODS</b>We successfully expressed a fragment of gG comprising residues 321-580 of HSV-2 with histidine tag (gG(321-580His)) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG(321-580His) as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference.</p><p><b>RESULTS</b>In testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG(321-580His)-ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%.</p><p><b>CONCLUSION</b>The study indicates that gG(321-580His) has a high diagnostic potential for HSV-2 virus serodiagnosis in humans.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Reference Values
/
Virology
/
Blood
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Herpes Genitalis
/
Chemistry
/
Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Qualitative research
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
Singapore medical journal
Year:
2015
Type:
Article
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