Analysis of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid and its clinical significance / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 297-299, 2002.
Article
in English
| WPRIM
| ID: wpr-244812
ABSTRACT
<p><b>OBJECTIVES</b>To study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).</p><p><b>METHODS</b>GCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.</p><p><b>RESULTS</b>The first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.</p><p><b>CONCLUSIONS</b>Calprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontium
/
Chemistry
/
Gingival Crevicular Fluid
/
Amino Acid Sequence
/
Leukocyte L1 Antigen Complex
/
Electrophoresis, Polyacrylamide Gel
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Stomatology
Year:
2002
Type:
Article
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