Cloning of full length cDNA sequence of the mouse ameloblastin / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 278-280, 2002.
Article
in English
| WPRIM
| ID: wpr-244818
ABSTRACT
<p><b>OBJECTIVE</b>Screening for special genes of matrix proteins of dentin and enamel of mouse dental germ.</p><p><b>METHODS</b>A cDNA library of dental germ of mouse was screened by differential display. The interesting clones were sequenced.</p><p><b>RESULTS</b>Six positive clones were isolated from the cDNA library. The sequence of one of the six positive clones was homologous with the ameloblastin sequence of rat. There are 497 homologous base pairs between the 526 base pairs sequenced by pTriplEX 3' primer of this clone and the 32-580 sequence of the rat ameloblastin gene; and there are 533 homologous base pairs between the 567 base pairs sequenced by pTriplEX 5' primer of this clone and the 1285-1854 sequence of the rat ameloblastin gene.</p><p><b>CONCLUSIONS</b>The full length cDNA sequence of the mouse ameloblastin was cloned.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Gene Library
/
Amino Acid Sequence
/
Cloning, Molecular
/
DNA, Complementary
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Stomatology
Year:
2002
Type:
Article
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