Study on the anti-tumor efficacy induced by heat shock protein 70-peptide complexes derived from tumor cells / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 153-156, 2002.
Article
in English
| WPRIM
| ID: wpr-244859
ABSTRACT
<p><b>OBJECTIVE</b>To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells.</p><p><b>METHODS</b>Cells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used.</p><p><b>RESULTS</b>HSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P < 0.01). CD8+ subset of T lymphocytes in the peripheral blood of immunized mice increased by 12%.</p><p><b>CONCLUSION</b>HSP70-PC induces anti-tumor immunity via activation of cytotoxic T lymphocytes (CTLs), and it possesses strong tumor vaccine effect. Our research adds more evidence to support the clinical use of HSP70-PC to fight human cancers.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Blood
/
Tumor Cells, Cultured
/
T-Lymphocytes, Cytotoxic
/
Cell Membrane
/
Chemistry
/
CD8 Antigens
/
HSP70 Heat-Shock Proteins
/
Therapeutic Uses
/
Drug Therapy
Limits:
Animals
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2002
Type:
Article
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