Immunoregulatory function of interleukin-6 on human Th17 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 496-498, 2011.
Article
in Zh
| WPRIM
| ID: wpr-244894
Responsible library:
WPRO
ABSTRACT
The aim of this study was to explore the regulatory function of interleukin-6(IL-6) on human Th17 cells. Human peripheral blood CD4(+) T cells were purified from healthy donors by anti-CD4 monoclonal antibody (mAb) conjugated microbeads. The experiment was divided into 2 groups. Test group in which CD4(+) T cells (1 × 10(6)/ml) were stimulated by human recombined IL-6 (20 ng/ml) for 4 days; control group in which CD4(+) T cells did not stimulated by IL-6. The concentrations of IL-17 protein in the supernatants were assayed by enzyme-linked immunosorbent assay (ELISA), and quantity of Th17 cells were detected by flow cytometry. The results showed that as compared to control group, IL-17 protein level in the supernatants of CD4(+) T cells significantly increased in IL-6 stimulated group: (337.05 ± 189.09 pg/ml; vs 15.07 ± 12.70 pg/ml) (p < 0.05). Furthermore, the percentage of Th17 cells in cultures of CD4(+) T cells stimulated by IL-6 was significantly higher than that in control group (4.05% ± 0.30% vs. 2.81% ± 0.44%)(p < 0.01). It is concluded that IL-6 promotes the expansion of Th17 cells in vitro.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Lymphocyte Activation
/
CD4-Positive T-Lymphocytes
/
Cells, Cultured
/
Interleukin-6
/
Cell Biology
/
Allergy and Immunology
/
Th17 Cells
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article