Methylation status of IEX-1 gene promotor CpG island in malignant hematopoietic cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 473-476, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-244899
ABSTRACT
This study was to purposed to explore the methylation status changes of IEX-1 gene promoter CpG island and its relevance with occurrence of hematologic malignancies. The methylation status of IEX-1 gene promoter CpG island in 9 NB4, HL-60 U937, Raji, CA46, Jurkat, K562, CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR, the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M. sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively. The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4, Molt4 and Raji cell lines, as well as in normal peripheral blood mononuclear cells treated by M. sssl enzyme; the partial methylation status was found in CA46, CEM, U937, K562, HL-60 and Jurkat cell lines; the unmethylation status was observed in untreated normal peripheral blood mononuclear cells. It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic malignancies to a certain extent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Leukocytes, Mononuclear
/
Gene Expression Regulation, Neoplastic
/
Promoter Regions, Genetic
/
CpG Islands
/
Hematologic Neoplasms
/
DNA Methylation
/
Cell Line, Tumor
/
Apoptosis Regulatory Proteins
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article
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