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Influence of TLR2 and TLR4 agonists on migration of cord blood CD34(+) cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 469-472, 2011.
Article in Chinese | WPRIM | ID: wpr-244900
ABSTRACT
This study was aimed to investigate the influence of TLR2 and TLR4 agonists on the migration and adhesion activity of umbilical cord blood (UCB) CD34(+) cells and to explore the underlying mechanism. The expression of TLR2 and TLR4 on UCB CD34(+) cells was detected with flow cytometry. The effect of TLR2 agonist (PAM3CSK4) and TLR2 agonist (LPS) on the migration and adhesion ability of UCB CD34(+) cells was evaluated with chemotaxis and adhesion assays. The results indicated that expression levels of TLR2 and TLR4 were (14.2 ± 3.8)%, (19.6 ± 4.1)% respectively. Compared with the control group, the migration activity of UCB CD34(+) cells toward SDF-1 decreased significantly in LPS group (p < 0.01). The adhesion activity was not altered significantly in LPS group. However, both the migration activity towards SDF-1 and the adhesion activity of UCB CD34(+) cells were not changed significantly in PAM3CSK4 group. Further study found that LPS did not affect the expression level of CXCR4 on CD34(+) cells, but could inhibit the spontaneous migration ability of CD34(+) cells. It is concluded that TLR4 activation can decrease the chemotaxis function of CD34(+) cells towards SDF-1, which may associate with the decreased spontaneous migration ability of CD34(+) cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Blood / Cell Movement / Cells, Cultured / Lipopolysaccharides / Antigens, CD34 / Cell Biology / Allergy and Immunology / Toll-Like Receptor 2 / Toll-Like Receptor 4 Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Blood / Cell Movement / Cells, Cultured / Lipopolysaccharides / Antigens, CD34 / Cell Biology / Allergy and Immunology / Toll-Like Receptor 2 / Toll-Like Receptor 4 Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2011 Type: Article