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Detection of bcr-abl fusion gene mRNA level in K562/A02 cell line by real-time quantitative RT-PCR / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 40-43, 2011.
Article in Chinese | WPRIM | ID: wpr-244989
ABSTRACT
This study was aimed to quantitatively analyze the mRNA level of bcr-abl fusion gene in K562/A02 cell line by real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) technique. After being cultured for a period of time, the K562/A02 cell line was collected and RNA was extracted using TRIzoL kit. The real-time quantitative reverse transcriptase polymerase chain reaction technology was used to detect the level of bcr-abl fusion gene and internal reference abl gene. The results showed that a fine reproducibility was obtained between 10(7) and 10(3) copies/ml, reproducible sensitivity of RQ-RT-PCR was 10(-5). The expression of bcr-abl fusion gene in K562/A02 cells was higher and the level of bcr-abl mRNA was more than 100% in K562/A02 cells. It is concluded that RQ-RT-PCR is a reliable, sensitive and reproducible method for detecting mRNA level of bcr-abl fusion gene, which may be useful in monitoring the chronic myeloid leukemia.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Fusion Proteins, bcr-abl / Sensitivity and Specificity / K562 Cells / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Genetics / Methods Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Fusion Proteins, bcr-abl / Sensitivity and Specificity / K562 Cells / Reverse Transcriptase Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction / Genetics / Methods Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2011 Type: Article