Inhibitory effect of arctigenin on lymphocyte activation stimulated with PMA/ionomycin / 药学学报
Acta Pharmaceutica Sinica
;
(12): 482-489, 2014.
Article
in English
| WPRIM
| ID: wpr-245058
ABSTRACT
This study investigated the effect of arctigenin (Arc) on the cell activation, cytokines expression, proliferation, and cell-cycle distribution of mouse T lymphocytes. Mouse lymphocytes were prepared from lymph node and treated with Phorbol-12-myristate-13-acetate (PMA)/Ionimycin (Ion) and/or Arc. CD69, CD25, cytokines, proliferation and cell cycle were assayed by flow cytometry. The results showed that, at concentrations of less than 1.00 micromol x L(-1), Arc expressed non-obvious cell damage to cultured lymphocytes, however, it could significantly down-regulate the expression of CD69 and CD25, as well as TNF-alpha, IFN-gamma, IL-2, IL-4, IL-6 and IL-10 on PMA/Ion stimulated lymphocytes. At the same time, Arc could also inhibit the proliferation of PMA/Ion-activated lymphocytes and exhibited lymphocyte G 0/G1 phase cycle arrest. These results suggest that Arc possesses significant anti-inflammatory effects that may be mediated through the regulation of cell activation, cytokines expression and cell proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Tetradecanoylphorbol Acetate
/
Lymphocyte Activation
/
T-Lymphocytes
/
Antigens, Differentiation, T-Lymphocyte
/
Antigens, CD
/
Ionomycin
/
Chemistry
/
Cytokines
Limits:
Animals
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2014
Type:
Article
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