The Effects of Interferon/Retinoic Acid on Cervical Cancer Cell Lines According to the Mutational Status of HPV-URR / 대한암학회지
Journal of the Korean Cancer Association
;
: 884-894, 2000.
Article
in Korean
| WPRIM
| ID: wpr-24520
ABSTRACT
PURPOSE:
We investigated the effects of all-trans-retinoic acid (ATRA) and/or interferon-gamma (IFN-gamma) on the growth of various cervical cancer cell lines and HPV E6/E7 expression. The relationships between the functional activities of HPV-URR and the growth inhibition were identified. MATERIALS ANDMETHODS:
Four groups of cell lines were included; i) with integrated form of HPV-16 DNA (SNU-17, CaSki), ii) episomal form of HPV-16 (SNU-523), iii) integrated form of HPV-18 (SNU-1160, HeLa) and iv) episomal form of HPV-18 (SNU-1245). The promoter activity of HPV-URR was confirmed by transient transfection assay in C33A using the HPV-18 URR-CAT reporter plasmid.RESULTS:
Selective mutation was detected in TEF-1 (transcriptional enhancer factor) binding site in SNU-17, and the activity of URR in SNU-17 was higher than that of the prototype. The proliferation was more inhibited in SNU-17 by IFN-gamma (10 ng/ml) than in SNU-902, CaSki and HeLa. The increase of the HPV-URR activity might play a role in the inhibition of growth by interferon-g. The expression of HPV-16 E6/E7 were significantly decreased by ATRA or IFN-gamma.CONCLUSION:
Point mutation at TEF-1 binding site of SNU-17 was related with the increased transcriptional activity of URR. Mutation in the HPV-URR and alteration of HPV-URR activity in SNU-17 might be related with significant growth suppression by IFN-gamma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Tretinoin
/
Binding Sites
/
DNA
/
Transfection
/
Cell Line
/
Uterine Cervical Neoplasms
/
Interferon-gamma
/
Point Mutation
/
Human papillomavirus 16
Type of study:
Prognostic study
Language:
Korean
Journal:
Journal of the Korean Cancer Association
Year:
2000
Type:
Article
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