Preliminary study on the gene expression profiles of oligodendrogliom as with cDNA array / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the gene expression profiles of oligodendrogliomas with gene cDNA array.</p><p><b>METHODS</b>(32)P tagged cDNA probes converted from the total RNA, which had been extracted from 2 fresh samples of oligodendroglioma and 1 of normal brain tissue, were hybridized with the Atlas array. After washing the membranes, the autoradiography was performed and the autoradiograms were analyzed through the special software.</p><p><b>RESULTS</b>As compared to the normal brain tissue, there were 63 co-upregulated genes and 4 co-downregulated genes in these 2 tumor samples. However, a significant quantitative difference existed between them. The expression trend of some genes differed from the known information.</p><p><b>CONCLUSION</b>cDNA array is effective for studying the gene expression profiles of oligodendrogliomas and provides new information for the further research on their molecular mechanisms.</p>