Nested coamplification polymerase chain reaction / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 347-349, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-245303
ABSTRACT
<p><b>OBJECTIVE</b>To develop a simple and reliable method for intensifying the hybridization signals of gene chips.</p><p><b>METHODS</b>The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.</p><p><b>RESULTS</b>With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.</p><p><b>CONCLUSION</b>Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
DNA Probes
/
Chemistry
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Edetic Acid
/
Fluorescent Dyes
/
Genetics
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2002
Type:
Article
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