Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 239-242, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-245324
ABSTRACT
<p><b>OBJECTIVE</b>To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy.</p><p><b>METHODS</b>Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies.</p><p><b>RESULTS</b>The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining.</p><p><b>CONCLUSION</b>Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Chemistry
/
Dystrophin
/
Fluorescent Antibody Technique
/
Muscle, Skeletal
/
Muscular Dystrophy, Duchenne
/
Muscular Dystrophy, Facioscapulohumeral
/
Diagnosis
/
Diagnosis, Differential
/
Metabolism
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2002
Type:
Article
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