Genetic polymorphism of D16S539, D7S820 and D13S317 loci in the Kazak ethnic population / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the genetic polymorphism of D16S539, D7S820 and D13S317 in Chinese Kazak ethnic population from Xinjiang.</p><p><b>METHODS</b>One hundred and two unrelated individuals and a sample of families (n=42) were investigated by multiplex amplification, 6% denaturing PAGE and silver staining. And, the obtained allele frequencies were compared with those of other populations.</p><p><b>RESULTS</b>Eight, seven, eight alleles were observed at the 3 STR loci respectively and the genotypes distributions were in accordance with Hardy-Weinberg equilibrium. The expected heterozygosities for these loci were 0.9439, 0.9356 and 0.9304; the calculated polymorphism formation content (PIC) was 0.9905; the discrimination power (DP), 0.9998; the paternity exclusion (PE), 0.9572. In addition, significant difference was found in comparison with other populations, and in the sample of families (n=42) no new mutations could be found.</p><p><b>CONCLUSION</b>The multiplex examination of 3 STR loci can be used in forensic identification and population genetics research.</p>