Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell growth and apoptosis of human chondrosarcoma cells HTB94 / 中华外科杂志
Chinese Journal of Surgery
;
(12): 772-775, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-245531
ABSTRACT
<p><b>OBJECTIVES</b>To construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.</p><p><b>METHODS</b>siRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells. Then the expression of the mRNA and protein of Sox9, cell growth and apoptosis in transferred HTB-94 human chondrosarcoma cells were checked.</p><p><b>RESULTS</b>The recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The expression of the mRNA and protein expression of Sox9 in transferred HTB-94 were significantly reduced. The cell growth of HTB-94 was inhibited, and the apoptosis of HTB-94 was remarkably increased.</p><p><b>CONCLUSION</b>siRNA (Sox9) expression plasmid could be transferred into HTB-94 human chondrosarcoma cells. And it can reduce the mRNA and protein expression of the HTB-94, inhibit the cell growth and cause the apoptosis of the tumor cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
RNA, Messenger
/
Tumor Cells, Cultured
/
Transfection
/
Chondrosarcoma
/
Apoptosis
/
RNA, Small Interfering
/
Cell Proliferation
/
SOX9 Transcription Factor
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2008
Type:
Article
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