Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line / 中华外科杂志
Chinese Journal of Surgery
;
(12): 768-771, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-245532
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.</p><p><b>METHODS</b>The prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.</p><p><b>RESULTS</b>The synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.</p><p><b>CONCLUSIONS</b>Cell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Prostatic Neoplasms
/
RNA, Messenger
/
Gene Expression
/
Cell Cycle
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Line, Tumor
/
Bcl-2-Associated X Protein
/
Genetics
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2008
Type:
Article
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