The Effect of Pantoprazole on Bone Turnover in Ovariectomized ICR Mice / 대한내과학회지
Korean Journal of Medicine
;
: 56-62, 2011.
Article
in Korean
| WPRIM
| ID: wpr-24571
ABSTRACT
BACKGROUND/AIMS:
Long-term exposure to proton pump inhibitors is associated with osteoporosis-related fractures; however, the mechanism is unknown. The purpose of this study was to evaluate the effect of pantoprazole on osteoporosis and bone turnover in ovariectomized ICR mice fed a calcium-free diet.METHODS:
Ovariectomized female ICR mice were divided into a pantoprazole group (n=10) and a control group (n=10). The mice in the pantoprazole group were given an intraperitoneal injection of pantoprazole at 20 mg/kg twice daily. After 4 weeks, the mice were humanely euthanized, and bone mineral density (BMD) and dry tibia weight were measured. Serum osteocalcin and CTX-1 levels were measured by enzyme-linked immunosorbent assay. The mRNA expression levels of cytokines that stimulate osteoclast differentiation were determined using RT-PCR. Serum calcium, phosphorus, and alkaline phosphatase (ALP) levels were also analyzed.RESULTS:
Serum osteocalcin concentration was significantly lower in the pantoprazole group compared with the control group (p=0.023). There was no difference in BMD, dry tibia weight, or serum ALP, calcium, phosphorus, or CTX-1 between the two groups. The expression of interleukin (IL)-1beta was lower in the pantoprazole group compared with the control group, but not significantly lower (p=0.058). The levels of tumor necrosis factor-alpha and IL-6 did not differ between the two groups.CONCLUSIONS:
Pantoprazole, a proton pump inhibitor, decreased serum osteocalcin and suppressed IL-1beta expression, suggesting that pantoprazole affects bone formation and resorption in ovariectomized ICR mice. Further studies using larger sample sizes are needed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoclasts
/
Osteogenesis
/
Osteoporosis
/
Phosphorus
/
Tibia
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Bone Density
/
Osteocalcin
/
Calcium
Limits:
Animals
/
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Medicine
Year:
2011
Type:
Article
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