Affinity maturation of a single-chain antibody against hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 192-195, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-245712
ABSTRACT
<p><b>OBJECTIVES</b>To obtain a single-chain antibody with high affinity against hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>A second single-chain antibody mutant library was established using an error-prone PCR and a phage display. Single-chain antibodies with high affinity for hepatocellular carcinoma were selected using ELISA.</p><p><b>RESULTS</b>The content of the second single-chain antibody mutant library was about 4.5 x 10(7). Two selected mutants, M90 and M116, were obtained after 3 rounds of panning and ELISA. Immunoassay showed that both M90 and M116 could bind to human HCC cells. The relative affinity of M90 was 1.7-fold higher than that of the original antibody, and M116 was 2-fold higher than that of the original antibody.</p><p><b>CONCLUSION</b>Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Immunoglobulin Variable Region
/
Immunoglobulin Fragments
/
Carcinoma, Hepatocellular
/
Peptide Library
/
Allergy and Immunology
/
Genetics
/
Liver Neoplasms
/
Antibodies, Neoplasm
/
Antibody Affinity
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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