Detecting the activity of antibodies induced by recombinant TGFbeta1 vaccine / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 183-186, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-245714
ABSTRACT
<p><b>OBJECTIVE</b>To measure the neutralization activity in vitro of the antibodies induced by recombinant TGFbeta1 vaccine and to evaluate the vaccine's anti-liver fibrosis activity.</p><p><b>METHODS</b>Balb/c mice were immunized with a fusion protein of the human TGFbeta1 epitope-inserted into a hepatitis B core antigen using a prokaryotic expression system. The antibody produced by the recombinant vaccine was determined using ELISA. The biological activity of the anti-TGFbeta1 antibody induced by the vaccine was measured by MTT using mink lung epithelial cell Mv-1-Lu as inhibiting cells. The fusion protein was used as a vaccine in a mice hepatic-fibrosis model.</p><p><b>RESULTS</b>A high titer of anti-TGFbeta1 antibody and a low of anti-HBc antibody were detected in the mice after the immunization. The serum antibodies induced combined with the fusion and antigenic peptide prevented the TGFbeta1 inhibiting activity in the Mv-1-Lu cell.</p><p><b>CONCLUSION</b>Recombinant fusion protein can be used as a cytokine vaccine to induce high titers of anti-TGFbeta1 antibodies. Our results show the potentiality of the fusion protein to be used as a vaccine for preventing liver fibrosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prokaryotic Cells
/
Blood
/
Recombinant Fusion Proteins
/
Carbon Tetrachloride
/
Carbon Tetrachloride Poisoning
/
Vaccines, Synthetic
/
Random Allocation
/
Therapeutic Uses
/
Allergy and Immunology
/
Transforming Growth Factor beta1
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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