Expression changes of interleukin-1 receptor associated kinase-4 during endotoxin tolerance development in kupffer cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 97-100, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-245740
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of endotoxin tolerance (ET) through observing the expression of interleukin 1 receptor associated kinase-4 (IRAK-4) during endotoxin tolerance development in Kupffer cells (KCs).</p><p><b>METHODS</b>Isolated KCs of Balb/c mouse were divided into two groups the non-endotoxin tolerance (NET) group and the endotoxin tolerance (ET) group, which were pretreated with 10 ng/ml lipopolysaccharide (LPS) for 24 h. Then, the two groups were treated with 100 ng/ml LPS. The expressions of IRAK-4 gene and protein level were determined by RT-PCR and Western blot. The activities of NF-kappaB of KCs and the TNFalpha level were estimated by ELISA at 0 h, 1 h, 3 h, 6 h and 12 h after LPS stimulation.</p><p><b>RESULTS</b>The ultimate level of IRAK-4, the activities of NF-kappaB and the TNFalpha level were evidently lower in the ET group than those in the NET group (t = 12.4, 17.4 and 138.9 respectively, P<0.01).</p><p><b>CONCLUSIONS</b>Pretreatment with LPS on KCs could induce endotoxin tolerance of KCs and inhibition of IRAK-4 expression may be one of the reasons for its development.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Lipopolysaccharides
/
Cell Biology
/
Endotoxins
/
Allergy and Immunology
/
Interleukin-1 Receptor-Associated Kinases
/
Genetics
/
Immune Tolerance
/
Kupffer Cells
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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