Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 11-14, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-245765
ABSTRACT
<p><b>OBJECTIVE</b>To determine the advantage of U1 small nuclear RNA as a ribozyme vector (U1-Rz) to inhibit HCV replication in vivo.</p><p><b>METHODS</b>The 3rd stem-loop was substituted by HCV core specific ribozyme to construct an U1-Rz eucaryotic expression plasmid. Then it was co-transfected with pCMV/T7-NCRC Delta-luc into Huh7 cell line mediated by lipofectin. The cell lysis supernatant was subjected to Western blot and lumenometer to determine the luciferase levels.</p><p><b>RESULTS</b>A U1 snRNA chimeric ribozyme was constructed successfully. Both Rz and U1-Rz inhibited luciferase expression in Huh7 by 48.64% and 87.46%, respectively.</p><p><b>CONCLUSION</b>Rz has more efficacy in cells when using U1 snRNA delivery system. U1 can be an efficient vector for HCV specific ribozyme.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virus Replication
/
Molecular Sequence Data
/
Base Sequence
/
Genetic Therapy
/
RNA, Small Nuclear
/
RNA, Catalytic
/
Hepacivirus
/
Genetic Vectors
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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