The direct antiviral effect of interferon-gamma on hepatitis C virus replicon / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 7-10, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-245766
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of interferon-gamma and interferon-gamma combined with interferon-alpha on HCV RNA replication and the possible mediators of interferon-gamma anti-HCV in vitro.</p><p><b>METHODS</b>An HCV replicon cell culture system was established and the cells were treated with interferon-gamma or interferon-gamma combined with interferon-alpha. HCV RNA levels in the cells were evaluated by semi-quantitative RT-PCR and real-time PCR, and the levels of NS5A protein were examined by Western blot.</p><p><b>RESULTS</b>Interferon-gamma could inhibit HCV RNA replication and NS5A protein expression effectively; The anti-HCV effects of interferon-gamma were both in time-dependent and does-dependent manners; Pretreating the cells with interferon-gamma could significantly enhance the antiviral effects of interferon-alpha; The expressions of IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69), ISGF3gamma and STAT1 were significantly increased after interferon-gamma treatment.</p><p><b>CONCLUSION</b>Interferon-gamma has a direct inhibitory effect on HCV replicon RNA replication and NS5A expression, and both are in a dose and time dependent manner; Interferon-gamma has a synergistic anti-HCV effect with interferon-alpha. IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69) and ISGF3gamma may mediate the anti-HCV effects of interferon-gamma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Pathology
/
Pharmacology
/
Replicon
/
Virus Replication
/
RNA, Viral
/
Tumor Cells, Cultured
/
Interferon-gamma
/
Interferon-alpha
/
Hepacivirus
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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