Effect of Shensu Yin on the expression of toll-like receptors and the downstream signaling components on RAW 264.7 cells / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influences of Shensu Yin to RAW 264.7 on the expression of TLR3, TLR4 and the factors of the downstream in RAW 264. 7 cells.</p><p><b>METHOD</b>RAW 264.7 cell line was stimulated with Lipopolysaccharide and POLY I C, respectively, and treated with the drug serum of Shensuyin simultaneously. 24 hours later, collected the supernatant and measured the inflammatory factors TNF-alpha and IFN-beta, extracted mRNA and measured the expression of TLR3, TLR4 and other correlated indexes of the downstream, analyzed and evaluated Shensu Yin's substance basis of pharmacodynamic actions.</p><p><b>RESULT</b>Shensu Yin drug serum depressed the expression of TLR4, MyD88, TRAF-6, TRAM and TRIF mRNA, as a result, it decreased the amount of TNF-alpha and IFN-beta.</p><p><b>CONCLUSION</b>Depressing the expression of TLR3, MyD88, TRAM and TRIF mRNA may be the elementary basis of Shensu Yin to play heat-clearing and detoxicating effect.</p>