Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 224-226, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-246004
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.</p><p><b>METHOD</b>The samples were separated using a Lichrosob C18 column with CH3CN-H2O (1090) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.</p><p><b>RESULT</b>The calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.</p><p><b>CONCLUSION</b>The contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenothiazines
/
Quality Control
/
Skin
/
Bufo bufo
/
Materia Medica
/
Chemistry
/
China
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Injections
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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