Simultaneous determination of six main constituents in Swertia of Qinghai Province and Sichuan Province by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2036-2038, 2006.
Article
in Zh
| WPRIM
| ID: wpr-246027
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside, mangiferin, swertianolin, isoorientin, 1,8-drihydroxy-3-methoxy-xthanone in Swertia from Qinghai province and Sichuan province by HPLC.</p><p><b>METHOD</b>The samples were separated on the column of Kromasil C18 (4. 6 mm x 250 mm, 5 microm) which eluted with methanol and water (content 0.02% phosphoric acid). The ratio of methanol increased from 20% to 80% during 20-50 min, and from 80% to 100% during 50-60 min, with detected wavelength 254 nm, flow rate at 1 mL x min(-1), column temperature 35 degrees C.</p><p><b>RESULT</b>Six compounds were base-isolated, the linear ranges of swertiamarin, gentiopicroside, mangiferin, 4-swertianolin, 5-isoorientin, 1,8-drihydroxy-3-methoxy-xthanone were excellent.</p><p><b>CONCLUSION</b>The method was rapid and precise, and can be use for controlling medicinal materials quality.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
/
Pyrans
/
Pyrones
/
Quality Control
/
Chemistry
/
China
/
Reproducibility of Results
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Chromatography, High Pressure Liquid
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Swertia
/
Iridoids
Country/Region as subject:
Asia
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article