Influence of Chinese herbal extract complex on expression of corticotropin-releasing factor and protein kinasec protein in hippocampus of middle cerebral artery occlusion rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1983-1986, 2006.
Article
in Zh
| WPRIM
| ID: wpr-246035
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the influence of one Chinese herbal extract complex (GETO) on the expression of corticotropin-releasing factor(CRF) and protein kinasec(PKC) proteins of the hippocampus in middle cerebral artery orilusion(MCAO) rats.</p><p><b>METHOD</b>All rats were subjected to MCAO by nylon thread, except the sham-group rats. Rats were divied into four groups: sham-group, cerebral ischemia model-group, GETO-group(6. 1 g x kg(-1) x d(-1) )and Duxil-group (7.3 mg x kg(-1) x d(-1)). Using immunohistochemistry technique we measured the expression quantity of CRF and PKC protein in hippocampus of MCAO rats at 2 h,6 h and 24 h after reperfusion, contrasted to Duxil.</p><p><b>RESULT</b>CRF: There were lots of positive and deeper dyeing neurons in hippocampus of model-group rats, while there were a few of positive and lighter dyeing neurons in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO -group and Duxil-group respectively( P <0. 01). PKC: There were a great number of denser positive granules in hippocampus of model-group rats, while there were a few of scattered positive granules in sham-group, GETO-group and Duxil-group. The positive expression areas of CRF protein in hippocampus of model-group was significantly bigger than that of sham-group, GETO-group and Duxil-group respectively( P < 0. 01). At the same time there was not significant difference about the expression of CRF and PKC protein between GETO group and Duxil-group.</p><p><b>CONCLUSION</b>The high expression of CRF and PKC induced by cerebral ischemia may be one important factor that resulted in the delayed neuronal death in hippocampus. GETO can down-regulate the expression of CRF and PKC induced by cerebral ischemia, which may be one of the mechanisms that the Chinese herbal extract complex, protect cerebral ischemic injury.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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Plants, Medicinal
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Protein Kinase C
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Corticotropin-Releasing Hormone
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Drugs, Chinese Herbal
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Immunohistochemistry
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Random Allocation
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Chemistry
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Rats, Sprague-Dawley
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article