Cloning and eukaryotic expression of HIV-1B gp120 genes from a patient with AIDS dementia complex / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 93-95, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-246171
ABSTRACT
<p><b>OBJECTIVE</b>To clone and express the HIV-1B gp120 genes isolated at different organizations from a patient died of AIDS dementia complex (ADC) in eukaryotic cells.</p><p><b>METHODS</b>Using the genomic DNA isolated from peripheral lymphnodes, choroid plexus and occipital white matter from a patient died of ADC as the template, HIV-1B gp120 gene was amplified with PCR. After sequenced, HIV-1B gp120 was inserted into pcDNA3.1 (+) and recombinant expressing vector gp120/pcDNA3.1 (+) was constructed succeffuly confirming with sequencing. Then expressing vector was transfected into eukaryotic cells U87 using liposome transfection and expression of HIV-1B gp120 gene was assayed with indirect immunofluorescence.</p><p><b>RESULTS</b>HIV-1B gp120 genes isolated from peripheral lymphnodes, choroid plexus and occipital white matter of the ADC patient were successfully cloned and recombinant expressing vector gp120/pcDNA3; 1 (+) could express envelope glycoprotein HIV-1B gp120 in U87 cells.</p><p><b>CONCLUSION</b>All the HIV-1B gp120 gene isolated at the different organizations of the same ADC patient could express in U87 cells, which may supply a valuable basis for studying the neurotoxicity and neurotoxic mechanism of HIV-1 gp120 protein.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Recombinant Proteins
/
HIV Envelope Protein gp120
/
AIDS Dementia Complex
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Toxicity
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Type:
Article
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