A study of immunoassay by using quantum dots to detect Hantavirus infection / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 486-488, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-246204
ABSTRACT
<p><b>OBJECTIVE</b>To develop a new method to detect anti-Hantavirus IgG antibodies (HV IgG) based on quantum dots (QDs) and indirect immune technique.</p><p><b>METHODS</b>The carbodiimide crosslinking method was used to couple protein G and goat antihuman IgG on the surface of water-solubility QDs. The coverglass covered HV antigen was used as carrier, and QDs-PG-IgG conjugates was used as labeled second antibody to detect the HV-IgG in the serum samples. The detecting conditions were optimized.</p><p><b>RESULTS</b>The optimum reaction time, pH and goat antihuman IgG concentration for conjugating the QDs with goat antihuman IgG were 6.0, 2h, and 20 microg/ml, respectively. The optimum working dilution of QDs-PG-IgG conjugates was 1 200. The detection limit of the serum samples was about 1 1280 dilution.</p><p><b>CONCLUSION</b>The method established in this study has been demonstrated to be a specific, sensitive, rapid test for detecting HV antibodies, laying the foundation of single molecule detection. The anti-fluorescence quenching ability of this method was significant improved.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Immunoglobulin G
/
Immunoassay
/
Hantavirus Infections
/
Quantum Dots
/
Diagnosis
/
Fluorescence
/
Methods
/
Antibodies, Viral
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article
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