Study on vector-specific and foreign gene-specific immune responses induced by rAd5 and rAAV2/1 vaccines / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 431-433, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-246221
ABSTRACT
<p><b>OBJECTIVE</b>To compare the foreign gene-specific and vector-specific immune responses in BALB/c mice immunized with rAd5 or rAAV2/1 expressing the same gene.</p><p><b>METHODS</b>BALB/c mice were immunized with rAd5-gag or rAAV2/1-gag once, HIV-1 Gag-specific and vector-specific cellular immune responses were analyzed by Elispot assay, HIV-1 P24-specific IgG and vector-specific IgG were tested by ELISA assay.</p><p><b>RESULTS</b>Mice immunized with rAd5-gag induced potent Gag-specific cellular immune responses and that were significantly higher than Ad5-specfic cellular responses, while rAAV2/1-gag elicited weak Gag-specific and AAV2/1-specific cellular responses. Both P24-specific and Ad5-specific IgG titers induced by rAd5-gag were high and in similar level. Higher level of P24-specific IgG was found in mice inoculated with rAAV2/1-gag than rAd5-gag. And the P24-specific IgG titers were higher than the vector-specific IgG titers in mice immunized with rAAV2/1.</p><p><b>CONCLUSION</b>rAd5 could elicit strong foreign gene-specific cellular and humoral immune responses, weak vector-specific cellular responses and strong vector-specific antibodies, rAAV2/1 could induce potent foreign gene-specific antibodies that were much higher than vector-specific IgG, while both foreign gene-specific and vector-specific cellular responses were very low.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Immunoglobulin G
/
Vaccines, Synthetic
/
Adenoviridae
/
HIV Core Protein p24
/
AIDS Vaccines
/
Dependovirus
/
Allergy and Immunology
/
Genetic Vectors
/
Mice, Inbred BALB C
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article
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