Anti-adenovirus neutralizing antibodies and Gag-specific cellular immune responses in Macaca fascicularis immunized with Ad5-HIVgag / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 413-415, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-246226
ABSTRACT
<p><b>OBJECTIVE</b>To observe the level of anti-adenovirus neutralizing antibodies and Gag specific cellular immune responses in Macaca fascicularis immunized with different dosage of recombinant adenovirus vaccine Ad5-HIVgag by repeated intramuscular injection.</p><p><b>METHODS</b>The Macaca fascicularis were randomly divided into four groups of 6. Different amount of the purified Ad5-HIVgag (0.99 x 10(11) VP, 4.94 x 10(11) VP, 24.68 x 10(11) VP) or PBS were administered in 3 weeks interval and five times. The level of anti-adenovirus neutralizing antibodies and Gag-specific cellular immune responses at different time points were detected by neutralization assay and Elispot assay respectively.</p><p><b>RESULTS</b>High level of anti-adenovirus neutralizing antibodies could be detected in three groups immunized with Ad5-HIVgag at 3 weeks after first immunization. The neutralizing antibodies reached peak at 8 weeks after primary immunization, and declined slightly at late time. Significant HIV-1 Gag-specific cellular immune responses were detected in all Ad5-HIVgag immunized groups at 5 weeks post first immunization. The Gag-specific cellular immune responses declined at 12 weeks and then increased with time.</p><p><b>CONCLUSION</b>Anti-adenovirus neutralizing antibodies could be induced in Macaca fascicularis immunized with Ad5-HIVgag by repeated intramuscular injection. And the Gag-specific cellular immune responses tended to increase with the injection times. The presence of anti-adenovirus neutralizing antibodies induced by vaccination with adenovirus vectors in Ad5-naive animals did not further reduce Gag-specific cellular immune responses.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Vaccines, Synthetic
/
Adenoviridae
/
Immunization
/
AIDS Vaccines
/
Allergy and Immunology
/
Gag Gene Products, Human Immunodeficiency Virus
/
Antibodies, Neutralizing
/
Immunity, Cellular
/
Macaca fascicularis
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article
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