Hypomethylation of TNF-alpha gene promoter in the patients with acute-on-chronic hepatitis B liver failure / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 368-370, 2011.
Article
in Zh
| WPRIM
| ID: wpr-246237
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The present study was designed to investigate the possible epigenetic alteration in the promoter of TNF-alpha in the patients with acute-on-chronic hepatitis B liver failure (ACHBLF).</p><p><b>METHODS</b>The methylation of TNF-alpha promoter in peripheral blood mononuclear cells (PBMCs) was measured by methylation specific PCR (MSP). The level of serum TNF-alpha was determined by enzyme-linked immunosorbent assay (ELISA). Model for End-stage Liver Disease (MELD) was performed for the evaluation of liver failure.</p><p><b>RESULTS</b>The serum level of TNF-alpha in patients with ACHBLF(44.9260 +/- 26.48523) was higher than that in CHB (18.92505 +/- 9.04461) and healthy controls (11.9172 +/- 5.04612) (P < 0.05). Moreover, the serum TNF-alpha level was significantly decreased in methylation group as compared to unmethylaiton group in patients with ACHBLF (P < 0.05). MELD was not significantly different between methylated and unmethylated group of ACHBLF patients (P > 0.05). In addition, the serum level of TNF-alpha was found to be positively correlated with serum total bilirubin (r = 0.891, P < 0.01) and MELD score (r = 0.792, P < 0.01), but to be negatively correlated with prothrombin activity (r = - 0.511, P < 0.05) in patients with ACHBLF.</p><p><b>CONCLUSION</b>The TNF-alpha methylation patten is stable for the liver failure, suggesting the effect of environment on methylation.</p>
Full text:
1
Index:
WPRIM
Main subject:
Blood
/
Promoter Regions, Genetic
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Tumor Necrosis Factor-alpha
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Liver Failure, Acute
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DNA Methylation
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Hepatitis B, Chronic
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Genetics
/
Metabolism
Type of study:
Prognostic_studies
Limits:
Adult
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Female
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Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article