Construction of recombinant plasmid expressing S1 gene of new type of reovirus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 361-363, 2011.
Article
in Zh
| WPRIM
| ID: wpr-246239
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant plasmid containing S1 gene of new type of reovirus, and to study the expression of protein sigma1 in Vero cells.</p><p><b>METHODS</b>The recombinant plasmid, named pC-S, was constructed by cloning S1 gene into vector pCAGGS/MCS. Then Vero cells were transfected with pC-S and collected at 24, 48, 72 h post transfection followed by SDS-PAGE and Western-Blot assay.</p><p><b>RESULTS</b>Results both SDS-PAGE and Western-Blot assay indicated that sigma1 protein could be expressed well and the highest expression level was 72 h post transfection.</p><p><b>CONCLUSIONS</b>Sigma1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene, and could give some implications for subsequent research on virus-host interactions.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Reoviridae
/
Viral Proteins
/
Vero Cells
/
Recombinant Proteins
/
Gene Expression
/
Chlorocebus aethiops
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article