Establishment of cellular immunity of enhanced hepatitis B vaccine / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 310-312, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-246255
ABSTRACT
<p><b>OBJECTIVE</b>To establish the method to detect the cellular immune response of enhanced hepatitis B vaccine and make verification preliminary.</p><p><b>METHODS</b>Immunized BALB/c mice with enhanced hepatitis B vaccine and detected the IFN-gamma spots forming cells (SFC) of mouse spleen cell by Elispot. Optimized the conditions of the experiment. Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared.</p><p><b>RESULTS</b>IFN-gamma SFC was higher in 5microg dose than in 2microg dose after immunization with enhanced hepatitis B vaccine and IFN-gamma SFC was declined after immunization 3 weeks ago. IFN-gamma SFC was higher in stimulus by peptide than by protein. Compared to normal hepatitis B vaccine, IFN-gamma SFC was higher in enhanced hepatitis B vaccine.</p><p><b>CONCLUSION</b>Established the detection method to evaluate the cellular immunity of enhanced hepatitis B vaccine and tested the repeatability.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Interferon-gamma
/
Hepatitis B Vaccines
/
Allergy and Immunology
/
Enzyme-Linked Immunospot Assay
/
Hepatitis B
/
Hepatitis B Surface Antigens
/
Immunity, Cellular
/
Methods
/
Mice, Inbred BALB C
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article
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