Study on ribotyping of Lyme borreliosis spirochete in Guizhou province / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 1129-1131, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-246387
ABSTRACT
<p><b>OBJECTIVE</b>To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.</p><p><b>RESULTS</b>There were two genospecies in the strains 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.</p><p><b>CONCLUSION</b>Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Restriction Fragment Length
/
DNA, Bacterial
/
Lyme Disease
/
Molecular Sequence Data
/
RNA, Ribosomal, 5S
/
RNA, Ribosomal, 23S
/
Base Sequence
/
Chemistry
/
China
/
Polymerase Chain Reaction
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2003
Type:
Article
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