Detecting caries susceptible population in children with gtfB oligonucleotide probe / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 378-386, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-246579
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quick, exact and inexpensive method to detect caries susceptibility in children.</p><p><b>METHODS</b>125 caries free children, aged 3-4 years, were randomly sampled. A combination of polymerase chain reaction (PCR) and gtfB oligonucleotide probe hybridization method was used to detect Streptococcus mutans in saliva. The participants were followed up for a year and the clinical examination results were compared with the laboratory results. The perspective study was used to evaluate the detecting approach.</p><p><b>RESULTS</b>When the combination of PCR and hybridization method was used to detect caries susceptibility of the sample, the predictive sensitivity, predictive specificity and predictive reliability were all increased to 69.2%, 46.8% and 54.3%, respectively as compared to only PCR, which were 56.4%, 44.2% and 48.3%, respectively. The samples with both the positive and negative results of hybridization detection had caries clinically, but the dmft index and prevalence were higher in the positive (dmft was 2.15 +/- 0.86, and the prevalence was 23.28%) than in the negative(dmft was 1.58 +/- 0.51, and the prevalence was 10.34%) which was statistically significant (P < 0.05).</p><p><b>CONCLUSION</b>The probe was found to be quite potential in detecting caries susceptibility, but the predictive specificity and predictive reliability values were not significant.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Streptococcus mutans
/
Oligonucleotide Probes
/
Predictive Value of Tests
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Dental Caries
/
Dental Caries Susceptibility
/
Diagnosis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Child, preschool
/
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2010
Type:
Article
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