Differentiation characteristics of human periodontal ligament cell population in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 185-189, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-246626
ABSTRACT
<p><b>OBJECTIVE</b>To explore the multi-differentiated capability of human periodontal ligament cell population (hPDLP), and provide a theoretical basis for the periodontal regeneration by tissue engineering technique.</p><p><b>METHODS</b>hPDLP was cultured from periodontium of human tooth by the outgrowth method. STRO-1 and CD 146 expression were investigated by flow cytometry. hPDLP was induced to odontogenic/osteogenic-like and adipogenic-like cell. The multilineage differentiation capacities of hPDLP were evaluated by alizarin red stain, oil red O stain, anti-CD146 and STRO-1 immunocytochemistry, and reverse-transcriptase polymerase chain reaction analysis.</p><p><b>RESULTS</b>hPDLP was isolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be induced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively. And the eighth generation of hPDLP had weaker potential into adipocyte-like cells than the first passage, however, there was no difference to the aspect of calcification ability between the two passages.</p><p><b>CONCLUSION</b>hPDLP cultured in vitro can differentiate into adipocytes and osteoblasts, and the first to third passage cells may have the predominance of differentiation potential.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Periodontal Ligament
/
Regeneration
/
In Vitro Techniques
/
Cell Differentiation
/
Cells, Cultured
/
Adipocytes
/
Tissue Engineering
/
Fibroblasts
/
Flow Cytometry
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2010
Type:
Article
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