Study of signal transduction pathway in the expression of inflammatory factors stimulated by lipopolysaccharides from Porphyromonas endodontalis in osteoblasts / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 135-138, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-246638
ABSTRACT
<p><b>OBJECTIVE</b>To quantify the interleukin (IL)-1beta mRNA and IL-6 mRNA expression induced by lipopolysaccharides ([PS) extracted from Porphyromonoas endodontalis (P. endodontalis) in osteoblasts, and to relate P. endodontalis LPS to the bone resorptive pathogenesis in the lesions of chronic apical periodontitis.</p><p><b>METHODS</b>MG63 cells was pretreated with PD98059 or SB203580 for 1 h and then treated with P. endodontolis LPS for 6 h. The expression of IL-1beta mRNA and IL-6 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) technique.</p><p><b>RESULTS</b>The production of IL-1beta mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with PD98059. Both of the production of IL-1beta mRNA and JL-6 mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with SB203580.</p><p><b>CONCLUSION</b>The synthesis of IL-1beta mRNA stimulated by Pendodontalis LPS in MG63 probably occur via extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen activated protein kinase (MAPK) signal transduction system. The synthesis of IL-6 mRNA stimulated by P.endodontalis LPS in MG63 probahly occur via p38MAPK signal transduction system.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pyridines
/
RNA, Messenger
/
Signal Transduction
/
Lipopolysaccharides
/
Interleukin-6
/
MAP Kinase Signaling System
/
Porphyromonas endodontalis
/
P38 Mitogen-Activated Protein Kinases
/
Imidazoles
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2010
Type:
Article
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