Experiment of oral mucosa epithelial cells cultured on small intestinal submucosa in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 76-80, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-246653
ABSTRACT
<p><b>OBJECTIVE</b>To explore an effective method to culture oral mucosa epithelial cells (OMECs) of canine in vitro, and to observe the biological characteristics of OMECs growing on small intestinal submucosa (SIS) in order to provide the experimental basis for epithelium tissue engineering.</p><p><b>METHODS</b>The primary OMECs were cultivated with DKSFM (defined keratinocyte serum free medium) containing 6% fetal bovine serum (FBS). The morphological characteristics and the growth curve of OMECs were observed. The expressions of OMECs marker (CK19) were examined by immunocytochemistry. The 2nd passage of OMECs were seeded on SIS, OMECs co-cultured with SIS were observed by hematoxylin-eosin staining, immunohistochemical staining, and scanning electron microscope (SEM).</p><p><b>RESULTS</b>OMECs were grown well in DKSFM. Immunohistochemical staining of the 2nd passage cultured canine OMECs with broadly reacting anti-cytokeratin anyibodies (CK19) was positive. OMECs formed a single layer on the surface of SIS, and eight days later the cells were polygong and arranged like slabstone.</p><p><b>CONCLUSION</b>Culture of canine OMECs in DKSFM containing 6% FBS is a simple and feasible method. SIS has good biocompatibility, it is a kind of good bioscafold in the tissue-engineered epithelium.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
In Vitro Techniques
/
Cells, Cultured
/
Coculture Techniques
/
Cell Culture Techniques
/
Tissue Engineering
/
Epithelial Cells
/
Intestine, Small
/
Mouth Mucosa
Limits:
Animals
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2010
Type:
Article
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