Your browser doesn't support javascript.
loading
Effect of Interferon Combined with Thalidomid on HEL Cell Apoptosis and JAK2V617F Mutation Gene Expression / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 998-1002, 2016.
Article in Chinese | WPRIM | ID: wpr-246828
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of interferon-α2b combined with thalidomid(THD) on the proliferation and apoptosis of HEL cells, and expression of JAK2V617F-mutated gene.</p><p><b>METHODS</b>The cell survival rates were assayed by CCK-8 after HEL cells were treated by interferon-α2b, thalidomid and thalidomid combined with interferon-α2b for 24, 48 and 72 hours, while the apoptosis and expression of JAK2V617F mutation gene were detected by flow cytometry and fluorescence quantitative PCR respectively after treatmemt for 48 hours.</p><p><b>RESULTS</b>IFN-α2b combined with THD could more obviously inhibit the proliferation of HEL cells than IFN-α2b or THD alone for 24 and 48 and 72 hours, and the differences between groups were statistically significant(P<0.05). In addition, the apoptosis-inducing effect of IFN-α2b combined with THD on HEL cells was more obvious than that of IFN-α2b used alone, the differences was statistically significant(P<0.05). Although IFN-α2b combined with THD can decrease the JAK2V617F mutation gene expression more obviously than IFN-α2b alone,but the difference was no statistically significant (P>0.05). And the remaining groups showed statistically significance(P<0.05).</p><p><b>CONCLUSION</b>IFN-α2b combined with THD can obviously inhibit the proliferation and induce apoptosis of HEL cells more than that of IFN-α2b alone, but the effect of reducing JAK2V617F mutation gene expression is not different.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Thalidomide / Gene Expression / Gene Expression Regulation, Neoplastic / Interferon-alpha / Apoptosis / Cell Line, Tumor / Janus Kinase 2 / Mutation Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2016 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Thalidomide / Gene Expression / Gene Expression Regulation, Neoplastic / Interferon-alpha / Apoptosis / Cell Line, Tumor / Janus Kinase 2 / Mutation Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2016 Type: Article