B-1 Cells Differ from Conventional B (B-2) Cells: Difference in Proliferation
Immune Network
;
: 155-160, 2004.
Article
in Korean
| WPRIM
| ID: wpr-24702
ABSTRACT
BACKGROUND:
B-1 cells differ from conventional B-2 cells both phenotypically and functionally. The aim of this study was to investigate the difference between peritoneal B-1 cells and splenic B-2 cells in proliferation.METHODS:
We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzyme- linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay.RESULTS:
Spontaneous Immunoglobulin M production occurred in peritoneal B-1 cells but not in splenic B-2 cells. LPS stimulated peritoneal B-1 cells secreted IgM at day 1, but splenic B-2 cells at day 2. In thymidine incorporation, peritoneal B-1 cells entered actively S phase after 24hours LPS-stimulation but splenic B-2 cells entered actively S phase after 48 hours.CONCLUSION:
IgM secretion and S phase entering occurred early in peritoneal B-1 cells compared to splenic B-2 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Thymidine
/
Ascitic Fluid
/
Immunoglobulin M
/
Immunoglobulins
/
S Phase
Limits:
Animals
Language:
Korean
Journal:
Immune Network
Year:
2004
Type:
Article
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