Lateral flow immunoassay for quantitative detection of ractopamine in swine urine / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 134-137, 2014.
Article
in English
| WPRIM
| ID: wpr-247072
ABSTRACT
A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT) to that of the control line (AC) was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration (IC50) of 0.59 ± 0.06 ng/mL. The limit of detection (LOD) of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenethylamines
/
Reagent Strips
/
Swine
/
Urine
/
Immunoassay
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2014
Type:
Article
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