Partial Nucleotide Sequence of Porcine Parvovirus (VRI-1 Strain): Identification of the Putative Defective Genomes
Journal of Bacteriology and Virology
;
: 201-212, 2004.
Article
in English
| WPRIM
| ID: wpr-24709
ABSTRACT
A porcine parvovirus, designated as VRI-1, was isolated from a 30-day-old piglet. Replicative form of viral DNA from ST cells infected with VRI-1 was directly cloned into pUC19. The cloned DNA fragment contained the entire nonstructural and structural protein genes, covering approximately 85% of the viral genome. The nucleotide sequence of VRI-1 showed 99.4~99.5% identity in the nonstructural protein (NS) and 99.0~99.2% identity in the structural protein with previously reported PPV strains, respectively. Among the cloned genes, two types of defective genomes with deletion of 100 and 247 nucleotides at almost similar location of 3' region within NS gene were also identified in this study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
DNA, Viral
/
Base Sequence
/
Genome, Viral
/
Genome
/
Clone Cells
/
Parvovirus, Porcine
/
Nucleotides
Type of study:
Diagnostic study
Language:
English
Journal:
Journal of Bacteriology and Virology
Year:
2004
Type:
Article
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