All-transretinoic acid regulates Th1/Th2 balance in CD4+ T cells when GATA-3 is deficient / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 774-777, 2013.
Article
in English
| WPRIM
| ID: wpr-247134
ABSTRACT
The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation. However, it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid (ATRA) on Th1-Th2 differentiation in CD4+ T cells under GATA-3 deficiency, which can induce Th1-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA. The expression of Th1 and Th2 cytokines were detected by qPCR and ELISA. The results would contribute to clarify the knowledge of the role of vitamin A in regulating Th1-Th2 balance under some special conditions, and help to explain the mechanism of immune regulatory function of vitamin A.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Tretinoin
/
CD4-Positive T-Lymphocytes
/
Cell Differentiation
/
Cells, Cultured
/
GATA3 Transcription Factor
/
Th1-Th2 Balance
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2013
Type:
Article
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