Influence of interphase nuclei preparation techniques on fluorescence in situ hybridization results in solid tumors / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a technology platform for the preparation of interphase nuclei for the fluorescence in situ hybridization (FISH) detection of solid tumor tissues.</p><p><b>METHODS</b>The centromere probe of chromosome 3 was labeled by the random primer technique, and then hybridized to interphase nuclei prepared by six different methods in order to study the influence on FISH detection.</p><p><b>RESULTS</b>Each method of slide preparation had its own characteristic, and could be used according to different needs. As regards to FISH, collagenase method got the best results. Whereas for frozen samples or small tissues, to prepare printing slides was more applicable.</p><p><b>CONCLUSION</b>The comparison of different slide preparation methods lays a technology foundation for the FISH application in cancer researches and clinical diagnosis of solid tumors.</p>