Novel partners of S100A8 identified in laryngeal cancer cell lines / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 266-270, 2007.
Article
in English
| WPRIM
| ID: wpr-247338
ABSTRACT
<p><b>OBJECTIVE</b>To explore mechanism of S100A8 in the oncogenesis and development of laryngeal cancer.</p><p><b>METHODS</b>Proteins interacting with S100A8 were isolated from laryngeal cancer cell lines Hep-2 by immunoprecipitation assay with anti-S100A8 antibody. The target bands were cut out and identified by maxtrix assisted laser desorption/ionization time of flight (MALDI-TOF). The peptide mass fingerprinting data of the proteins identified were analyzed based on the Mascot database. The NF-kappa B binding sites of the proteins were predicted by P-Match software. The binding ability of one of the proteins to S100A8 was confirmed by co-immunoprecipitation and immunocytochemistry methods.</p><p><b>RESULTS</b>Four proteins interacting with S100A8 were obtained, which were hypothetical protein LOC80154, MHC class I HLA-B, similar to T-box 1 isoform C and sarcolemmal associated protein 1. The four genes were predicted to have NF-kappa B binding sites. MHC class I HLA-B, which is one of targets in NF-kappa B pathway, was first confirmed to have the binding ability to S100A8.</p><p><b>CONCLUSION</b>The novel partners of S100A8 identified in the study might be involved in NF-kappa B pathway. The binding ability of MHC class I HLA-B to S100A8 implies that S100A8 might function as a new member with other proteins including HLA-B in NF-kappa B pathway. These findings provide a new clue to further study on the molecular mechanism of S100A8 in the genesis of laryngeal carcinomas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Binding Sites
/
Carcinoma, Squamous Cell
/
HLA-B Antigens
/
Signal Transduction
/
Laryngeal Neoplasms
/
NF-kappa B
/
Calgranulin A
/
Cell Line, Tumor
/
Genetics
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Type:
Article
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